FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_04.3510000007ed1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_04.3510000007ed1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8234594
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC111958513.596116578425116TruSeq Adapter, Index 4 (100% over 51bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC305280.37072866008937416TruSeq Adapter, Index 4 (98% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC251160.3050059298612658TruSeq Adapter, Index 4 (100% over 50bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG190150.2309160597353069Illumina Single End PCR Primer 1 (96% over 30bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG184090.22355686266985353TruSeq Adapter, Index 4 (100% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC151590.18408922164225705TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1322950.042.76793345
CTCGTAT1366800.041.43370442
CGTATGC1367450.041.41565744
TCGTATG1372050.041.3538843
TCTCGTA1364000.041.3521641
CAGTCAC1411550.041.31670827
CTCCAGT1419300.041.28450424
CCAGTCA1412450.041.28401626
TCCAGTC1419750.041.2413125
AGTCACT1388150.041.24005528
ACTCCAG1424050.041.19103623
GTCACTG1379350.041.1883529
CACTGAC1371200.041.1804731
ACTGACC1365200.041.1785232
AATCTCG1365200.041.08672739
GACCAAT1358900.041.06527335
TCACTGA1380250.041.06042530
CCAATCT1357900.041.0507837
ATCTCGT1372300.041.0348340
ACCAATC1359200.041.0330436
Produced by FastQC (version 0.11.2)