H5LWJBCXY_l01n01_mb_biochip-dam_03.3510000007ece4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5LWJBCXY_l01n01_mb_biochip-dam_03.3510000007ece4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8847785
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC	744480	8.414309344090075	TruSeq Adapter, Index 3 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC	15358	0.17358016723959727	TruSeq Adapter, Index 3 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG	13624	0.15398204183306893	TruSeq Adapter, Index 3 (100% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC	10524	0.11894502409360082	TruSeq Adapter, Index 3 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG	10496	0.11862856070756693	Illumina Single End PCR Primer 1 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	88470	42.60778	45
CGTATGC	91685	41.165245	44
CTCGTAT	92060	41.029102	42
TCTCGTA	91895	40.90934	41
TTAGGCA	90470	40.860226	34
TCGTATG	92665	40.856155	43
TAGGCAT	90395	40.835777	35
CACTTAG	92055	40.804382	31
CGTCTGA	96020	40.773716	16
ATCTCGT	92040	40.749546	40
CATCTCG	91690	40.738228	39
AGTCACT	93860	40.73402	28
GCATCTC	90955	40.703785	38
TCTGAAC	96180	40.687172	18
CAGTCAC	95755	40.64454	27
TCACTTA	93315	40.622314	30
CTGAACT	96465	40.599617	19
ACTCCAG	96430	40.567688	23
CTCCAGT	96400	40.545303	24
GTCTGAA	96755	40.463978	17