FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_02.3510000007ecca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_02.3510000007ecca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16361874
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC6309223.85604974100155TruSeq Adapter, Index 2 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG287000.17540778030682794Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC762650.041.97566245
CGTATGC781200.041.03941344
CTCGTAT783800.040.84836242
TCGTATG789750.040.67773443
TATCTCG783600.040.53158639
TCTCGTA788000.040.4678941
AGTCACC809600.040.43822528
CAGTCAC819250.040.42053627
CGTCTGA837200.040.36819516
GTATCTC780300.040.35986738
CGATGTA780850.040.23874334
CCAGTCA825650.040.1371926
TCCAGTC837650.040.12625525
CTCCAGT839600.040.0759424
GATGTAT782550.040.00793535
TCTGAAC844600.040.00118618
ATGTATC781950.039.9753336
GTCTGAA845250.039.97308717
ACTCCAG843600.039.95792823
CCGATGT789200.039.92976833
Produced by FastQC (version 0.11.2)