FastQCFastQC Report
Mon 6 Mar 2017
H5LWJBCXY_l01n01_mb_biochip-dam_01.3510000007eca1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LWJBCXY_l01n01_mb_biochip-dam_01.3510000007eca1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9124356
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1466931.607707985089578TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC183600.041.3967445
CGTATGC187750.040.52964444
CTCGTAT191050.039.72358342
CAGTCAC200200.039.4581227
TCGTATG193800.039.3224543
CCAGTCA202700.039.18235826
CGTCTGA206300.038.97848516
TCCAGTC205650.038.9156925
CATCACG193150.038.9064333
TCACGAT192050.038.684335
TCTCGTA194650.038.6768141
CTCCAGT207950.038.5285524
CACGATC192450.038.4285336
ACGTCTG210050.038.34687415
ACTCCAG209600.038.31112323
GTCACAT200650.038.29315629
ACGATCT196000.037.9506137
AGTCACA204000.037.90696328
GATCTCG197550.037.8129239
CACGTCT213800.037.79003514
Produced by FastQC (version 0.11.2)