FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n02_3036_d5.352000000b08ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n02_3036_d5.352000000b08ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10485932
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG330100.31480272807414733No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC211620.20181324845516832No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC171870.16390531618934778No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC144350.13766062949864638No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC126820.12094299295475118No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA120670.11507799211362424No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT114430.10912716199189543No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT107580.1025946000794207No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA107380.1024038683447499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT77950.040.8111081
AACCGTG81150.039.099132
ACAACCG39200.036.8355062
ACCGTGT99150.031.9493873
CCGTGTT105400.030.0999164
CGTGTTC114150.027.8758645
TTCACTA125750.025.0308449
GTTCACT136500.023.3377958
TGTTCAC140450.023.1208787
CACAACC68350.022.1236611
GTGTTCA147800.021.971096
AGACTCA90450.020.3728874
ACAGACT85100.020.2607822
AACTCAC126950.018.4061015
ACTCACC130600.017.8487686
CAGACTC119600.015.8045213
GAACTCA151500.015.7682964
CACAGAC118200.015.4081211
AGAACTC154800.015.3707913
CACTAGC118850.014.99963610-11
Produced by FastQC (version 0.11.2)