FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n02_3030_d5.352000000b09ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n02_3030_d5.352000000b09ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11741503
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG204200.17391299904279717No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC146330.12462629358439034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT55650.032.9842221
AACCGTG59250.030.7837522
ACCGTGT73000.025.0480563
ACAACCG31050.024.6287842
CCGTGTT81050.022.9118354
CGTGTTC91450.020.4100935
CGCGTAT3003.6743586E-919.0214731
TTCACTA98850.018.6828299
GTTCACT109950.016.926248
TGTTCAC120250.016.0291567
AACTCAC107650.015.9709495
AGACTCA93700.015.6115954
GTGTTCA128550.015.5481846
ACAGACT92650.014.7647392
ACTCACC114100.014.1469556
CACAACC65150.013.13839151
GAACTCA138600.012.5416314
CACTAGC85750.012.26335410-11
AGAACTC143150.011.7780443
GCGCGAA28500.011.74394314-15
Produced by FastQC (version 0.11.2)