FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n02_3025_d3.352000000b08fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n02_3025_d3.352000000b08fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10388104
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG432060.41591805395864345No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC227480.21898125009145075No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC187660.18064894229014264No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC171370.16496754364415295No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT151460.1458013897434989No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA148290.1427498222967348No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT146450.14097856548220927No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA139270.13406681334726722No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC128990.12417087853567889No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA125060.12038770501334989No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA111520.1073535651934174No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC107600.10358001806681952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT90850.046.21731
AACCGTG95450.043.739652
ACAACCG42850.043.4507832
ACCGTGT114200.036.6382373
CCGTGTT126400.033.214674
CGTGTTC135750.030.996935
CACAACC67050.028.2971631
TTCACTA161050.026.2945549
TGTTCAC164250.026.0713087
GTTCACT166950.025.3937428
GTGTTCA168200.025.2050276
ACAGACT82400.021.1544272
AGACTCA88300.020.3308644
AACTCAC127000.018.6978235
ACTCACC131400.017.9565416
GAACTCA146500.017.1815764
CACAGAC111700.016.8581851
CAGACTC114650.015.8653423
AGAACTC150900.015.8308233
CACTAGC151850.015.19438510-11
Produced by FastQC (version 0.11.2)