FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n02_3014_d3.352000000b08c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n02_3014_d3.352000000b08c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716395
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG311950.2910960262289697No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC189690.17700915279811913No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC123380.11513200101340049No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC117070.10924382686528446No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT115120.10742418509209486No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA113100.10553922284499591No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC108810.10153601094397882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT66150.044.1390461
AACCGTG69200.042.0759242
ACAACCG32150.034.27572
ACCGTGT89750.032.4914173
CCGTGTT96950.030.1274264
CGTGTTC104600.028.3326765
TTCACTA127950.022.9676659
TGTTCAC135800.022.16447
GTTCACT138550.021.587418
GTGTTCA141400.021.3537526
CACAACC63550.018.931691
AGACTCA87550.018.0101224
ACAGACT86450.017.6917252
AACTCAC116550.017.1962975
ACTCACC120000.016.2602446
CGCGAAT2951.218199E-616.1199021
GAACTCA140600.014.93040854
CACTAGC118900.014.014955510-11
CACAGAC119750.013.97820951
AGAACTC144700.013.8837413
Produced by FastQC (version 0.11.2)