FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n02_3013_d5.352000000b09c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n02_3013_d5.352000000b09c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8504209
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG181340.2132355872251023No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC100800.118529542253724No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC89190.10487747890485757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT51100.034.710091
AACCGTG54800.032.1564332
ACAACCG29350.030.9100532
ACCGTGT68700.026.0618633
CCGTGTT75100.024.2203084
TTCACTA77000.023.5523999
CGTGTTC83700.021.8452035
GTTCACT91200.020.4058048
TGTTCAC94000.019.7979747
GTGTTCA100200.018.3834296
CACAACC53500.017.865291
AACTCAC71800.015.6101985
AGACTCA62750.015.5910134
CGTATCA22000.015.55904848-49
GCCGTAT23500.014.76617946-47
ACTCACC79750.014.4061256
GTATACG5702.1827873E-1114.159136
ACAGACT66150.014.1452582
GAACTCA90750.013.2402054
CACTAGC75600.013.15602610-11
Produced by FastQC (version 0.11.2)