FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n02_3013_d3.352000000b08aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n02_3013_d3.352000000b08aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8880777
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG153900.17329564744165965No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT95250.1072541287772455Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG69850.037.8587049
AACCGTG46250.033.273692
CAACCGT47650.032.1340071
GAGCGTC85800.030.6548658
AAGAGCG95600.029.7968446
GCCGTAT39900.028.59065846-47
CGTATCA39400.028.53534748-49
CGCCGTA40700.027.78727344-45
AGAGCGT94750.027.7592327
ACAACCG23800.026.1433962
ACCGTGT59700.025.695193
ATCGGAA111950.025.4858461
CCGTATC42600.025.2722846-47
TCGGAAG119350.024.2758872
CCGTGTT65800.023.8905284
CGTGTTC69200.022.9912385
GTAGATC66850.022.48813430-31
GTCGCCG59100.022.1052242-43
GGTCGCC61200.022.04532642-43
CGGAAGA131300.021.8833753
Produced by FastQC (version 0.11.2)