FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n01_3036_d5.351000000b08e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n01_3036_d5.351000000b08e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10485932
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG326200.31108345924806685No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC202460.1930777350072459No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC167270.1595184862919195No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC142560.13595358047334274No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC124640.11886401704683952No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA116430.11103447933860339No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT116120.11073884514986364No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT112420.10721030805845393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT77300.041.9456021
AACCGTG80600.040.2472952
ACAACCG39500.035.110482
ACCGTGT98050.033.1813473
CCGTGTT108900.030.1806954
CGTGTTC117000.028.1327955
TTCACTA130850.025.2331759
GTTCACT137600.023.9263048
TGTTCAC143050.023.4464827
GTGTTCA150700.022.3459326
CACAACC65300.022.0521431
ACAGACT85850.020.6357172
AGACTCA91450.019.9953044
AACTCAC119250.017.2860955
ACTCACC122350.016.7316536
CACAGAC114600.016.7262461
CAGACTC117000.016.3189133
GAACTCA140850.015.34283354
CACTAGC122950.014.70235610-11
ATTAGGC31800.014.6374195
Produced by FastQC (version 0.11.2)