FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n01_3030_d5.351000000b09ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n01_3030_d5.351000000b09ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11741503
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG205860.17532678737977583No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC143420.12214790559607232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT57050.035.3469471
AACCGTG58950.033.83722
ACAACCG32000.026.1211092
ACCGTGT76050.026.1039353
CCGTGTT82900.024.0042674
CGTGTTC91300.021.9010185
TTCACTA96650.020.3007569
GTTCACT108850.018.7673958
TGTTCAC120850.017.0611027
GTGTTCA126600.016.544666
AACTCAC104000.015.9384135
ACTCACC109100.014.7580156
AGACTCA91500.014.2219254
CGCGTAT3405.1909155E-614.0213011
ACAGACT89850.013.5845092
GAACTCA132850.013.3344844
CACTAGC86350.012.65404810-11
CACAACC71550.012.3928331
AGAACTC136050.012.1830443
CGGGCAT33450.011.8290321
Produced by FastQC (version 0.11.2)