FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n01_3025_d3.351000000b08fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n01_3025_d3.351000000b08fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10388104
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG440460.4240042263727818No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC222200.21389851314542094No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC189340.1822661767729703No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC177490.1708588978315966No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT153850.14810209832323587No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT152860.14714908514585529No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA146190.14072827919320022No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA140740.13548189351974144No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC134770.12973493526826455No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA126260.12154287250108393No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC109380.10529351650695835No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA104630.10072097853467774No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC103940.10005675722923066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT94300.047.36471
AACCGTG100700.044.0476762
ACAACCG43250.042.93352
ACCGTGT124600.035.713073
CCGTGTT136600.032.4019284
CGTGTTC143600.031.188035
CACAACC70100.027.4719981
TTCACTA166350.026.7302479
TGTTCAC170350.026.437247
GTTCACT171600.026.1062418
GTGTTCA175700.025.9225436
ACAGACT85000.021.5103472
AGACTCA90050.021.2533284
AACTCAC126500.018.5467225
ACTCACC129950.017.6157656
CAGACTC118450.016.7990463
CCGGTAT9400.016.734511
CACAGAC117950.016.6100431
ATTAGGC36250.016.5079545
GAACTCA148150.016.412474
Produced by FastQC (version 0.11.2)