FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n01_3014_d3.351000000b08c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n01_3014_d3.351000000b08c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716395
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG316560.2953978460107154No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC191370.17857684417194403No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC122990.11476807265876258No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT120490.1124351985905708No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC116810.10900120796219252No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA116710.10890789299946484No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC110200.10283308892589345No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT107220.10005230303660886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT66100.043.780221
AACCGTG70950.040.6317862
ACAACCG33400.031.4250092
ACCGTGT93750.031.0034353
CCGTGTT99700.029.2484514
CGTGTTC107750.026.976285
TTCACTA127900.022.7689639
TGTTCAC140150.021.4906447
GTTCACT139100.021.1064248
GTGTTCA145350.020.5533926
CACAACC61300.018.8211271
AGACTCA86200.017.1349474
AACTCAC118300.016.7416865
ACAGACT84950.016.4925672
ACTCACC122900.016.0377736
GAACTCA140550.014.73284
AGAACTC142300.014.0843633
CACTAGC122950.013.54293410-11
CACAGAC117150.013.0226211
CAGACTC117950.012.9654483
Produced by FastQC (version 0.11.2)