FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n01_3013_d5.351000000b09c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n01_3013_d5.351000000b09c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8504209
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG182550.21465841208747338No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC100330.1179768747451997No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC87440.10281967435184153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT49600.035.3706321
AACCGTG51000.034.3600852
ACAACCG27500.028.493732
ACCGTGT65850.027.044163
CCGTGTT77150.022.959954
TTCACTA79850.022.2501349
CGTGTTC82100.021.6919675
GTTCACT90300.019.5174068
TGTTCAC97150.018.3368367
GTGTTCA103750.017.668926
CACAACC50300.016.1123031
AGACTCA65200.015.6598964
AACTCAC72800.015.2649465
TCGTATG25350.014.98626644-45
ACAGACT63900.014.4921162
ACTCACC79050.014.2382726
TATCTCG26300.013.18065640-41
CACTAGC78650.012.62360910-11
GAACTCA96850.012.3565984
CTAGCAA82000.012.25012612-13
Produced by FastQC (version 0.11.2)