FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l02n01_3013_d3.351000000b08ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l02n01_3013_d3.351000000b08ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8880777
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG154260.1737010173771957No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC149770.16864515345898226TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT47600.032.7547041
AACCGTG47700.032.2605062
TCGTATG52600.029.43268642-43
TATGCCG52500.028.35836444-45
ACTCGTA19650.028.27534740-41
ACAACCG26300.027.8105452
CTCGTAT53450.027.40892840-41
CGTATGC54900.027.1183542-43
ATCGGAA115400.026.3188251
ACCGTGT60150.026.0569063
TCGGAAG121750.025.2784562
ATCACGA61750.024.12216832-33
CCGTGTT64850.024.0219554
GTATGCC65850.023.51065844-45
ATGCCGT64300.023.20195846-47
CGGAAGA131700.023.152283
CGTGTTC69850.022.7106465
AGCACAC135900.022.4772769
AACTCGT25600.022.1707238-39
AGAGCAC138800.021.9391967
Produced by FastQC (version 0.11.2)