FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n02_neg_lp_control_cb10-12_blood.352000000b09df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n02_neg_lp_control_cb10-12_blood.352000000b09df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378681
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG155504.106358650156728Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT46061.2163271988824367Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT29700.7843012984543719Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG13150.3472579823122892Illumina Single End PCR Primer 1 (97% over 49bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGG12390.3271883194562178Illumina Single End PCR Primer 1 (97% over 49bp)
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG12110.31979423314082306No Hit
ATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT4610.12173834969274931Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGTGGTCGCCGT3910.10325313390426243Illumina Single End PCR Primer 1 (96% over 28bp)
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG3880.10246091037047014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA3200.080.20971
GATCGGA22700.068.680131
AGAGCGG5900.067.618557
GGAGAGC3750.062.0916335
GAGAGCG4050.057.4922566
TCGGGAG4450.055.5279432
GGGAGAG4600.053.717254
CGGGAGA4700.052.5743333
GAGCGGC7700.050.5780148
TCGGAAG43000.048.1821672
AGCGGCG8100.048.0803349
AAGAGCG42500.047.630926
CGGAAGA44200.047.0890663
ATCGGAA44300.046.887911
GAAGAGC43400.046.752675
AGCGTCG39400.045.9269269
CGTATCA30700.045.7330348-49
GCCGTAT30850.045.66467746-47
GAGCGTC39750.045.642028
AGAGCGT40100.045.4805267
Produced by FastQC (version 0.11.2)