FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n02_3025_d5.352000000b089d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n02_3025_d5.352000000b089d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14010673
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG598850.4274241501461064No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC407820.2910780945355016No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC309600.22097439573388086No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA281120.20064703529944639No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT242370.17298954875329686No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC240010.17130511860493783No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT220450.15734433313802984No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA211090.1506637118716567No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC192820.13762365305363988No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC181760.1297296710871776No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC174810.12476916704857789No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG174720.124704930305632No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA173260.12366286758673192No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG164800.11762461374981772No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA160830.11479105964431545No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA159950.11416296704662224No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG156500.11170055856702958No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC155210.11077983191813841No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC146310.10442753178237763No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT142430.10165821441982122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT108850.053.7548561
AACCGTG117550.049.6114652
ACCGTGT146550.039.891413
CCGTGTT161400.036.2211044
ACAACCG45750.035.5299952
CGTGTTC176050.033.4499445
TGTTCAC217050.027.2701267
GTGTTCA226200.026.4120926
TTCACTA222500.026.3662769
GTTCACT231000.025.4175598
ACAGACT109050.022.707652
AGACTCA120950.021.888174
CACAACC81350.020.8008231
AACTCAC211150.019.3132935
ATTAGGC49950.019.1258475
ACTCACC217600.018.5442356
CACAGAC149500.017.86831
CAGACTC152250.017.7941683
GAACTCA239650.017.4924774
TATTAGG54150.017.4668564
Produced by FastQC (version 0.11.2)