FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n02_3022_d3.352000000b0873.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n02_3022_d3.352000000b0873.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10122090
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG305690.3020028472380704No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC194970.19261832289576558No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA158490.1565783351066825No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC142400.14068240847492958No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT129760.12819486884625605No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT121590.12012341324765934No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA110670.1093351274292167No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC105620.10434603920731786No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC105610.10433615982470024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT66350.046.9942631
AACCGTG70500.043.753392
ACCGTGT87650.035.5719763
CCGTGTT98050.031.7504464
ACAACCG27400.030.0090082
CGTGTTC108350.028.7321765
TTCACTA134050.023.2817829
TGTTCAC139150.022.5313387
GTTCACT140250.022.2191418
GTGTTCA147950.021.58796
AACTCAC110550.019.3468325
ACTCACC112150.019.11326
AGACTCA78050.016.6243954
GAACTCA134500.016.3965244
ACAGACT73750.015.8536472
AGAACTC140150.015.4981273
CACAACC57900.015.3512921
CACTAGC128300.013.75353610-11
TCACTAG126200.013.4827913
ATTAGGC34450.013.3825435
Produced by FastQC (version 0.11.2)