FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n02_3021_d5.352000000b08b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n02_3021_d5.352000000b08b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12459777
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG335750.26946710201956264No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC218180.17510746781423134No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA151830.12185611347618822No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC149630.12009043179504737No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT130470.10471294951747531No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC128510.10313988765609529No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT125490.10071608825743832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT75900.044.96481
AACCGTG78850.042.9783632
ACCGTGT101300.033.6412243
CCGTGTT111750.030.4102944
ACAACCG36550.030.169122
CGTGTTC126800.026.9508045
TTCACTA146150.023.2720959
GTTCACT157650.021.6653088
TGTTCAC162700.021.2557097
GTGTTCA176000.020.0649556
AACTCAC126900.017.41625
ACTCACC131850.016.7983996
CACAACC72250.015.9866851
AGACTCA96100.015.7277214
ACAGACT95950.014.7120632
ATTAGGC39100.014.3439035
GAACTCA158500.014.3037934
CGCATAT5057.6033757E-1014.1185441
CACTAGC136950.013.85588210-11
AGAACTC164700.013.6787633
Produced by FastQC (version 0.11.2)