FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n02_3008_d5.352000000b098f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n02_3008_d5.352000000b098f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13481688
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG258070.19142261710848077No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC207590.15397923464776814No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC148420.11009007180703188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT67700.036.1580281
AACCGTG69250.035.003182
ACCGTGT89300.027.1973483
CCGTGTT100100.024.5478574
CGTGTTC111100.022.2884985
ACAACCG33800.021.514532
TTCACTA130350.018.9494329
AACTCAC127750.017.93265
TGTTCAC147050.017.7026717
GTTCACT143200.017.5150978
ACTCACC133700.017.134556
GTGTTCA155050.016.4917836
GAACTCA158250.015.0470424
ATTAGGC36800.014.594455
AGACTCA100750.013.5864054
AGAACTC170250.013.4560343
TATTAGG40850.013.0311594
CTTTATT166500.012.1327941
TCACTAG116050.012.0818653
ACAGACT99900.011.9416782
Produced by FastQC (version 0.11.2)