FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n02_3005_d5.352000000b0965.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n02_3005_d5.352000000b0965.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859657
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG443400.34479924309023174No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC352870.27440078689501596No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC255290.1985200693922085No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA219950.17103877653968533No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT195710.15218912915017874No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC185080.1439229677743349No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT171170.13310619404545548No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA157620.1225693655748361No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC152080.11826131910050167No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC144690.1125146650489978No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG139410.10840880126118449No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG137660.10704795625575395No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA133180.10356419304185173No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA130540.10151126114794506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT92150.049.6713681
AACCGTG98800.046.1327972
ACCGTGT125250.036.4285283
ACAACCG41050.033.692092
CCGTGTT136750.033.3998264
CGTGTTC150100.030.5242165
TGTTCAC186700.025.0619667
TTCACTA185600.024.7750139
GTGTTCA193650.024.2241086
GTTCACT190950.024.1806628
AACTCAC181950.020.5575265
AGACTCA107300.020.0653444
ACTCACC184100.019.9043836
ACAGACT101350.019.7426972
CACAACC77650.019.1592311
GAACTCA207100.018.9790124
AGAACTC209250.017.8754673
ATTAGGC43300.016.2450855
CACAGAC137000.015.8551671
CTCAGAA178850.015.5734171
Produced by FastQC (version 0.11.2)