FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n01_neg_lp_control_cb10-12_blood.351000000b09d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_neg_lp_control_cb10-12_blood.351000000b09d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378681
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT166484.396312463524708TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG150873.9840921514414505TruSeq Adapter, Index 20 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCCTATCTCGTATG16300.43044145336048023TruSeq Adapter, Index 20 (97% over 40bp)
CGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCG11590.3060623585550899No Hit
ATCGGAAGAGCACACGTCTGAACTCCCGTCACGTGGCCTTATCTCGTATG7500.1980558834480737TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTAACTCGTATG7150.18881327555383026TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGGCTTATCTCGTATG7080.18696475397498158TruSeq Adapter, Index 20 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATT7030.18564438141866108TruSeq Adapter, Index 20 (97% over 43bp)
ATCGGAAGAGCACACGTCTGAAATCCAGTCACGTGGCCTTATCTCGTATG6130.16187767540489223TruSeq Adapter, Index 20 (97% over 40bp)
CTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCT4060.1072142515732239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22950.069.537251
GAGCACA45300.051.2035148
AGCACAC45550.051.0268369
TCGGAAG46050.050.0136532
AGAGCAC46600.049.877097
GAAGAGC46500.049.3710445
CGGAAGA46800.049.1106833
AAGAGCA47200.048.638856
ATCGGAA47800.048.4354931
GGAAGAG47750.048.12094
TATCTCG39700.046.0700640-41
GTCACGT42600.045.80905528-29
TCGTATG41600.045.6788644-45
CACGTGG43000.045.50235730-31
GCCTTAT36950.045.25606536-37
CTTATCT38200.044.7700638-39
ACGTCTG44000.044.6153814-15
CAGTCAC42450.044.56688326-27
GTATGCC42550.044.5473546-47
CTGAACT43150.044.4490318-19
Produced by FastQC (version 0.11.2)