FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n01_3025_d5.351000000b0890.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_3025_d5.351000000b0890.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14010673
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG607910.4338906489359933No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC409040.2919488592732126No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC305170.21781252049776623No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA288260.20574315023982073No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC244770.1747025285651874No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT243680.17392455023395378No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT231240.16504560487565445No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA204950.14628133851957006No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC185940.13271311092622032No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC182170.1300223051383756No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC180590.12889459342888096No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG174760.12473347996916351No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG173900.12411966220323607No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA171550.1224423694707599No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA162320.11585453461086415No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC157930.11272120903828102No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG156110.11142219934759737No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA156010.11135082518876858No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC151300.10798910230793339No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT144620.10322130849817136No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC141650.1011014959809568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT114750.054.0657161
AACCGTG122650.049.9512632
ACCGTGT157600.038.9642753
ACAACCG46450.037.72812
CCGTGTT169350.036.2836844
CGTGTTC183350.033.5088125
TGTTCAC225800.027.6929177
TTCACTA231900.026.7390829
GTGTTCA235750.026.6050226
GTTCACT241100.025.6202228
ACAGACT113350.023.0863022
AGACTCA127200.022.0255744
CACAACC84700.021.9966741
TATGCCG45900.020.6938673
AACTCAC216050.020.1017235
ACTCACC220550.019.6700336
CGTATGC48500.019.4530241
ATTAGGC51400.019.0434445
CACAGAC152800.018.4301031
GAACTCA242500.018.1718254
Produced by FastQC (version 0.11.2)