FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n01_3022_d3.351000000b0876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_3022_d3.351000000b0876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10122090
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG317140.313314740335247No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC195090.19273687548717705No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA160300.158366503360472No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC144920.1431720128945702No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT129340.12777993477631597No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT128640.12708837799308245No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC108880.10756671794066246No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA108550.10724069831428094No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC108520.10721106016642808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT68100.046.3849531
AACCGTG72850.043.093472
ACCGTGT94350.033.6259163
CCGTGTT105650.030.0243454
ACAACCG32400.029.1707522
CGTGTTC114150.027.6825685
TGTTCAC141000.023.0511887
TTCACTA138150.022.9420019
GTTCACT147750.021.4835138
GTGTTCA153950.020.8963096
AACTCAC111150.018.8534035
ACTCACC112050.018.701976
CGTATGC29400.018.6363661
TATGCCG29750.018.5187033
CGCGTAT2002.6998177E-416.6754951
CACAACC61850.016.5618721
AGACTCA80300.016.5580464
GAACTCA133850.016.3904254
ACAGACT76800.015.5221572
ATTAGGC33100.015.360895
Produced by FastQC (version 0.11.2)