FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n01_3021_d5.351000000b08b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_3021_d5.351000000b08b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12459777
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG344090.2761606407562511No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC213940.17170451766512354No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA160540.12884660776834128No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC148280.11900694530889278No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT134810.1081961579248168No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT129050.10357328225055713No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC128800.10337263660497294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT76750.044.8807451
AACCGTG80050.043.07422
ACCGTGT102300.033.9842223
CCGTGTT112650.030.8151024
ACAACCG35700.029.0021482
CGTGTTC125900.027.7393995
TTCACTA150800.023.0642819
TGTTCAC157200.022.6995687
GTTCACT159650.021.9940748
TATGCCG38150.021.2884223
GTGTTCA170500.021.1799356
CGTATGC40450.020.1408481
AACTCAC125950.017.5801755
ACTCACC129950.016.7465236
CACAACC71350.016.092491
AGACTCA96600.015.5341444
ATGCCGT53200.015.4422964
ACAGACT92450.014.7440532
ATTAGGC40250.014.638325
GAACTCA160350.014.5408544
Produced by FastQC (version 0.11.2)