FastQCFastQC Report
Sat 23 Dec 2017
H5LW5BCX2_l01n01_3021_d1.351000000b0918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_3021_d1.351000000b0918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16120999
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG1965611.2192854797646224No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC1221040.7574220431376493No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA1124610.6976056508656815No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC841170.521785281420835No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT826540.5127101614484314No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT789050.48945477882605165No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG702350.4356739926601323No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC644920.4000496495285435No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA595370.3693133409412159No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG583710.3620805385571949No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA544890.33800014502823306No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC543500.33713791558451184No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC521190.32329882285831046No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG504970.3132374116517221No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC489070.30337449930987526No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC487250.30224553701665763No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA485540.3011848087081948No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG482800.29948516217884513No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG452030.28039825571603844No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA448680.2783202207257751No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA441660.2739656518805069No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC436060.27049192174752945No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA432210.2681037322811074No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA430040.2667576618545786No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA424650.2634141966015878No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT422460.2620557199960126No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC403020.24999691396296225No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC398450.24716210205087163No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA388350.24089698163246584No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC386060.23947647413165896No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT380910.2362818830272243No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC370220.2296507803269512No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT363680.22559395977879534No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC361460.22421687390465067No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA358940.2226536953448108No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG354880.22013524099840212No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA353920.21953974440417742No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA353870.21950872895656154No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA352440.21862168715474764No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC339880.210830606713641No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC336890.20897588294621194No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG336020.20843621415769584No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG328560.20380870937340795No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG323820.20086844493942344No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT321910.19968365484049716No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC319790.19836859986158425No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC315290.1955772095761559No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG313670.19457230907340173No Hit
CTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCT312650.19393959394203797No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT309360.19189877748891368No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG305180.1893058860682269No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT304940.18915701191967074No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA291620.18089449667480284No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC290260.18005087649965115No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG289840.17979034673967786No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA282150.17502017089635696No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC276270.17137275425673063No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT275200.17070902367775098No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA275090.17064078969299606No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA274800.17046090009682402No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC271880.1686495979560572No Hit
ATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCT270550.16782458704947503No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC270120.16755785419997857No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC268930.16681968654672083No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT267730.16607531580393994No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC264010.1637677665013192No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA261890.16245271152240626No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC256030.15881770106182627No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA255130.15825942300474058No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT252970.156919555667735No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT252690.1567458691610861No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC244920.15192606860157984No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG242450.15039390548935583No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA240420.1491346783161515No Hit
CTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGA238130.14771417081534463No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC237510.14732957926490783No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA237080.14706284641541134No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC236610.14677130120782217No Hit
AACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGG233450.14481112491849915No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA233420.14479251564992965No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG232430.1441784097871354No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC230920.1432417432691361No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG230460.14295640115107008No Hit
CAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCGG230420.1429315887929774No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT229290.14223063967685873No Hit
CCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGC220550.1368091394336046No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC216350.13420384183387146No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC216020.1339991398796067No Hit
GTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCA214550.13308728571970013No Hit
CAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAA212070.13154891951795294No Hit
CTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCGGTGACGG211370.13111470325133076No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC208260.12918554240962363No Hit
CTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGG207460.1286892952477697No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG202300.12548850105381187No Hit
TGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTG202210.12543267324810328No Hit
CTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTCCACCTG196020.12159296083325855No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA195370.12118976001425222No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC195350.12117735383520586No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC194860.12087340244857034No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA194460.12062527886764338No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT193680.12014143788483579No Hit
CAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGC191920.11904969412875717No Hit
CCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCG190490.11816265232694326No Hit
CGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGA187410.11625210075380564No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC187130.11607841424715677No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC184420.11439737698637657No Hit
GTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTG183980.11412444104735692No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG183140.1136033815274103No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC180890.11220768638469612No Hit
GATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCC180220.11179207938664347No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC179230.11117797352384923No Hit
CACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAA178500.11072514798865753No Hit
CCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGAT176340.10938528065165191No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA174720.10838038014889771No Hit
CTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTA174640.10833075543271233No Hit
CCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGAC173750.10777868046514982No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA172330.1068978417528591No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT170680.1058743319815354No Hit
GGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTA170590.10581850417582682No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG169730.10528503847683385No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA169420.10509274270161545No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG168280.10438559049597361No Hit
CCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGT163120.10118479630201577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT302900.064.555591
AACCGTG328150.059.6309172
ACAACCG94100.053.955382
ACCGTGT417850.046.8526653
CACAACC111200.046.103431
ACAGACT115900.046.06062
CACAGAC127600.042.8663941
CCGTGTT457300.042.7932624
CGTGTTC500550.039.1003845
GTGTTCA568150.034.5735666
CGCCCTT28450.034.4993321
TGTTCAC573150.034.30497
AGACTCA163300.033.179774
CAGACTC174900.031.0115153
CTCAGAA279450.030.6898061
TCAGAAA286100.029.5660112
GTTCACT679050.028.8429858
CTGAATA42350.028.3512941
CGGGTCT36750.028.0041351
TTTGAGC60750.028.0009946
Produced by FastQC (version 0.11.2)