FastQCFastQC Report
Fri 22 Dec 2017
H5LW5BCX2_l01n01_3008_d5.351000000b0982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_3008_d5.351000000b0982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13481688
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG261700.19411515827988307No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC214300.1589563562070269No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC150240.1114400511271289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT66600.040.2051731
AACCGTG67250.039.4054572
ACCGTGT92050.029.0468523
CCGTGTT102450.026.0020374
CGTGTTC114150.023.6439765
ACAACCG31400.022.3844552
TTCACTA133950.019.936029
TGTTCAC148850.018.5788577
GTTCACT146250.018.486788
AACTCAC127550.018.2542385
TATGCCG38900.017.4582583
CGTATGC39800.017.3582061
GTGTTCA159600.017.297826
ACTCACC132850.017.1683186
GAACTCA156000.015.4021864
AGACTCA100700.014.1936294
AGAACTC167550.013.8321553
CGCGTAT3701.2497581E-512.8771021
ATGCCGT54750.012.6626664
ACAGACT101700.012.32813552
Produced by FastQC (version 0.11.2)