FastQCFastQC Report
Fri 22 Dec 2017
H5LW5BCX2_l01n01_3005_d5.351000000b0968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LW5BCX2_l01n01_3005_d5.351000000b0968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12859657
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG460040.35773893502758275No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC351170.27307882317545484No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC248120.1929444929985302No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA225180.1751057590416292No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT192800.1499262383125771No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC189210.14713456198715097No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT181740.141325697878256No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC151220.11759256098354723No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA150860.11731261572528723No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC143410.11151930413074004No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG140720.10942749095096393No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG137780.10714127134184061No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA133720.10398411092924174No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC132320.10289543492489729No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA132300.10287988241054953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT90400.049.8536071
AACCGTG95700.047.0448462
ACCGTGT126700.035.5342673
CCGTGTT139650.032.23514
ACAACCG40250.032.2116052
CGTGTTC152500.029.6929425
TGTTCAC191450.024.2722647
TTCACTA190150.023.9884249
GTGTTCA193350.023.861916
GTTCACT196150.023.3757638
AACTCAC176150.021.201675
AGACTCA105500.020.9746654
TATGCCG40250.020.8844473
ACAGACT100550.020.4513242
ACTCACC182500.020.3598276
CGTATGC41550.020.2943971
GAACTCA201600.018.961254
CACAACC75600.018.6527921
AGAACTC208000.017.9462973
ATTAGGC45750.017.6558255
Produced by FastQC (version 0.11.2)