FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n02_8265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n02_8265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58196001
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1903140.3270224701522017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGCG156850.061.4263237
GAGCGGG171350.058.810659
AGAGCGG185100.052.6398668
GGGGAGA1084650.035.985574
CGCCGTT18050.034.141533145
GGGAGAG1134950.032.882975
GGGGGAG1455900.027.4272123
AGAGGGG895250.026.869158
GAGAGGG956200.025.3000587
CGTCGTT20000.024.287683145
GGAGAGC448550.024.129866
GGAGAGG1058350.023.6728346
GAGGGGG1269000.023.0747769
GGGGGGA1888150.022.5581252
GCGTCGG39250.020.688055145
CGGAAGC112350.018.713984145
TCGGAAC38000.017.171185145
GTGGGGA839600.016.4754582
CGCCGTG23300.015.558058145
TGGGGAG845600.014.4890883
Produced by FastQC (version 0.11.2)