FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n02_64141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n02_64141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68009734
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2765300.40660356060207503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGCG219800.074.1449057
GAGCGGG244450.071.384759
AGAGCGG256850.065.003018
GGGGAGA1536500.043.4033244
GGGAGAG1580150.040.465055
CGCCGTT26100.033.333424145
AGAGGGG1281250.032.438988
GAGAGGG1289650.032.3789947
GGGGGAG2185950.032.2726823
GGAGAGC563250.031.7522566
GGAGAGG1409850.030.677296
GAGGGGG1866850.028.1661629
GGGGGGA3030850.024.855632
GTGGGGA1106700.020.3929982
CGTCGTT22800.020.350935145
GAGGGCG152900.020.2457147
CGGAAGC145400.019.945034145
TGGGGAG1036250.019.6449243
AGGGCGG156300.019.5273028
GCGTCGG57500.018.030485145
Produced by FastQC (version 0.11.2)