FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n02_63766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n02_63766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71210919
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2351440.3302077873759781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGCG239150.064.508487
GAGCGGG259300.062.0971579
AGAGCGG257850.061.462168
GGGGAGA1535500.041.2596054
GGGAGAG1590300.037.635635
AGAGGGG1257550.030.6352238
GGGGGAG2086300.029.6756173
GAGAGGG1302450.029.4616077
GGAGAGC604200.028.388376
GGAGAGG1440900.027.5710436
GAGGGGG1813650.025.8308779
GGGGGGA2830200.023.5029092
GTGGGGA1155100.020.3293782
CGCCGTT33500.018.611881145
TGGGGAG1111650.018.4494553
GAACGGG86350.015.7001919
CGGAAGC148700.014.334186145
GAGGGCG180150.014.2054847
AGGGCGG182650.013.8525748
CGTCGTT30100.013.247467145
Produced by FastQC (version 0.11.2)