FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n02_53651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n02_53651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72437857
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1730310.23886819291189135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGCG183800.052.695857
GAGCGGG195600.051.5933389
AGAGCGG210150.046.5031368
CGCCGTT25450.031.90611145
GGGGAGA1246050.031.8889624
GGGAGAG1338200.028.2137975
CGTCGTT25800.024.44799145
GGGGGAG1613250.023.8623773
AGAGGGG1036700.023.3359178
GAGAGGG1105000.021.8997677
GGGGGGA2024100.020.6956312
GCGTCGG47450.020.627243145
GAGGGGG1424650.020.462059
GGAGAGC528050.020.3873126
GGAGAGG1248750.020.1157516
CGGAAGC144350.017.779932145
TCGGAAC50050.016.5137145
GTGGGGA1011250.014.9765932
CGCCGTG26700.014.120033145
GAACGGG73800.013.7529199
Produced by FastQC (version 0.11.2)