FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n02_3728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n02_3728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68032977
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2207460.32446911738111944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGCG151050.047.8026737
GAGCGGG167700.045.3489079
AGAGCGG178050.041.8575678
CGCCGTT21300.033.356747145
GGGGAGA1080550.027.3801774
GGGAGAG1190350.023.8798855
CGTCGTT20950.022.840052145
GGGGGAG1428650.021.0135143
AGAGGGG903850.020.2288678
GGGGGGA1704150.018.8847262
GAGAGGG1005250.018.4043357
GAGGGGG1260500.018.2035149
GCGTCGG46750.017.989271145
CGGAAGC126450.017.487116145
GGAGAGG1116150.017.2574446
GGAGAGC487850.017.1782556
CGCCGTG25400.015.413357145
CGGTGGG181450.013.42516145
TCGCCGG92850.012.33708145
GTGGGGA905900.012.1805732
Produced by FastQC (version 0.11.2)