FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n02_13013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n02_13013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56492192
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1658390.29356092254306576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG140900.043.0149779
GAGAGCG127350.041.3286557
CGCCGTT24900.036.686733145
AGAGCGG180450.030.6140068
CGTCGTT19500.028.256401145
CGCCGTG29250.026.521357145
GGGGAGA925050.024.3808274
GCGTCGG55300.022.54972145
GGGAGAG1002350.022.0808435
GGGGGAG1307100.020.4771543
CGGAAGC155400.020.201086145
CGTATCG8450.018.875732145
AGAGGGG804800.018.6197838
GGGGGGA1557150.018.3161142
GAGGGGG1143550.018.1949629
TCGGAAC36500.018.075335145
GAGAGGG841600.017.95177
CGGTGGG189450.016.838211145
GGAGAGG961400.016.370496
TCTCGGG131000.015.440834145
Produced by FastQC (version 0.11.2)