FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n01_8265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n01_8265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58196001
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC8044031.3822307137564316TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG675270.11603374603007516No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590510.10146917139547097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG63900.023.257118145
CACGTCG20700.020.66258145
ATGCCGG63750.020.014067145
CGGAAGC131400.019.364872145
GAGCACA7091300.017.5846049
AGAGCAC7225100.017.2508438
CGGAAGA7452550.016.6396774
GAAGAGC7587300.016.4433566
TCGGAAG7637100.016.2517663
AAGAGCA7774450.016.093417
ATCGGAA7780350.015.9674642
GATCGGA7878000.015.5777241
GGAAGAG8172250.015.3080755
CATCGGA50650.013.7430491
TCGGAAC41350.012.79824145
AGGGGGG2026300.012.03632970-74
GAGCACC317550.011.985332145
AAGGGGG2096550.011.69249970-74
CTGAACG66200.011.169791145
CACACGG93200.010.889677145
Produced by FastQC (version 0.11.2)