FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n01_64141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n01_64141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68009734
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC14781752.1734756380608693TruSeq Adapter, Index 10 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710770.10451003969520009No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA8383900.027.9483289
AGAGCAC8537850.027.459428
CGGAAGA8775050.026.6552854
GAAGAGC8957550.026.2203856
TCGGAAG8986400.026.0435773
ATCGGAA9145950.025.6170162
AAGAGCA9187550.025.6107087
GATCGGA9254100.024.942031
GGAAGAG9609050.024.4720695
CGGAAGC159250.019.893131145
AATCGGA87550.019.8772891
CATCGGA65300.019.432421
ATGCCGG135700.018.590906145
CACGTCG27700.017.011192145
CTTATCG169000.015.656955145
AGGGGGG2920200.015.53108470-74
TATCGGA58950.015.0064531
AAGGGGG3032250.014.98633570-74
AAAGGGG3399400.013.48290870-74
AAAAGGG3744750.012.24838670-74
Produced by FastQC (version 0.11.2)