FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n01_63766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n01_63766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71210919
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC12313481.7291561705586191TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG773750.10865608966512566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA6755150.029.5380429
AGAGCAC6853350.029.1241158
CGGAAGA6892050.028.881884
TCGGAAG7040200.028.2821873
ATCGGAA7151700.027.858522
GAAGAGC7190400.027.7851166
GATCGGA7204400.027.292571
AAGAGCA7467200.026.8074867
GGAAGAG7809050.025.6507825
AGGGGGG2504750.015.38925870-74
AAGGGGG2583000.014.98086970-74
CGGAAGC164900.014.903173145
CATCGGA72650.014.6717641
ATGCCGG157900.014.507902145
AAAGGGG2945450.013.25555370-74
TATCGGA62450.013.0042771
CGTCTTC3042350.012.51433150-54
TGAATCG195950.012.430646145
GCCGTCT3087950.012.26508845-49
CCGTCTT3099200.012.22348650-54
Produced by FastQC (version 0.11.2)