FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n01_53651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n01_53651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72437857
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC7830781.0810341890705022TruSeq Adapter, Index 9 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG25250.021.245853145
CGGAAGC168900.018.499138145
ATGCCGG80050.017.84057145
GAGCACA8370350.014.4975099
AGAGCAC8509300.014.2504838
CGGAAGA8747900.013.7896624
GAAGAGC8960400.013.5362116
TCGGAAG8958700.013.4756553
ATCGGAA9120000.013.2436882
AAGAGCA9209200.013.2296067
GATCGGA9219550.012.956641
CTGAACG88900.012.721159145
GGAAGAG9656100.012.6217675
GTCTTCG72350.010.92174145
CACACGG118700.010.50465145
TCGGAAC51800.010.356328145
GAGCACC385000.010.337499145
AGGGGGG2350800.010.09795570-74
AAGGGGG2473950.09.62811170-74
ACCGCGC45350.08.9539331
Produced by FastQC (version 0.11.2)