FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n01_3728.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n01_3728.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68032977
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC6020240.8849002741714508TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG702800.10330284385467947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG119500.025.418404145
CACGTCG21700.022.382946145
ATCATCG203200.019.942991145
CGGAAGC148100.018.551813145
GAGCACA7369500.012.9514959
GTCTTCG77250.012.856545145
AGAGCAC7506750.012.714658
CGGAAGA7651950.012.3981114
CTGAACG78850.012.227906145
TCGGAAG7828500.012.1351053
GAAGAGC7882350.012.1312696
ATCGGAA7976850.011.91671852
AAGAGCA8147700.011.8016047
GATCGGA8068400.011.6388651
GAGCACC367550.011.281888145
GGAAGAG8517950.011.2626475
TCGGAAC46550.010.122688145
GTCGTCG13352.568413E-99.774475145
GCCGTCG63100.09.535664145
CATCGGA57050.09.1510641
Produced by FastQC (version 0.11.2)