FastQCFastQC Report
Mon 5 Dec 2016
H5LVHBGXY_n01_13013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LVHBGXY_n01_13013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56492192
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC6569011.1628173323492208TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG90000.029.078205145
CACGTCG23150.027.557165145
ATGCCGG72850.024.877878145
CGGAAGC146200.020.280508145
CACGGCG36250.018.598495145
CATCGGA54200.014.3148411
GAGCACA7442650.013.7212919
AGAGCAC7604150.013.417438
CGGAAGA7888750.012.8725154
GAAGAGC8006700.012.733556
GAGCACC322350.012.616475145
TCGGAAG8102150.012.5405733
AAGAGCA8163800.012.5180327
CACACGG96900.012.419016145
ATCGGAA8256500.012.3114352
GATCGGA8354100.012.0013581
GGAAGAG8573700.011.9692585
GCCGTCG28700.011.114083145
CTGAACG66450.010.364098145
AGGGGGG1918650.010.35913970-74
Produced by FastQC (version 0.11.2)