FastQCFastQC Report
Sat 16 Nov 2024
H5LN2BGYW_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LN2BGYW_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338508349
Sequences flagged as poor quality0
Sequence length6
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAA106905763.1581424894190717No Hit
TAAAAA40518451.196970477085633No Hit
AAAAAT34301431.0133111960556103No Hit
AAAATA30463850.8999438297458359No Hit
AATAAA25385750.7499298045378491No Hit
AAATAA23907290.7062540723330875No Hit
GAAAAA22951650.6780231585957132No Hit
CAAAAA22770910.672683851587956No Hit
ATAAAA22622180.6682901637974076No Hit
AAAATT19991270.5905694810499341No Hit
AAAAAC16432640.4854426795836577No Hit
TAAAAT15616770.46134076297184623No Hit
AAAACA14613570.4317048617314901No Hit
ACAAAA14256910.4211686371138811No Hit
TAAATA13316370.39338379804629275No Hit
AATTAA13084570.38653610874454386No Hit
AAAAAG12929620.3819586736396862No Hit
AACAAA12535210.370307262347612No Hit
TTAAAA12182730.35989452065183775No Hit
AAACAA11969100.35358359802227507No Hit
TATAAA11337750.33493265479251144No Hit
TAATAA10730210.3169850915553046No Hit
TAAATT10427830.3080523724394166No Hit
ATTAAA10195890.3012005473460272No Hit
AAAATG10054630.29702753358086303No Hit
AAATTA9989210.29509493723004154No Hit
AATAAT9582650.28308459830631827No Hit
AAATAT8960390.26470218611949214No Hit
AAAAGA8755630.25865329543171767No Hit
GAAAAT8569990.25316923571654654No Hit
AAAAGG8464910.2500650286767373No Hit
ATAAAT8416170.2486251823585007No Hit
CAAAAT8192800.24202652679624156No Hit
AAAATC8183670.24175681409854977No Hit
AATATA8178670.24160910725424972No Hit
AAAACT7932540.23433809013673693No Hit
AAAAGT7865860.23236827166115184No Hit
ATATAA7744230.22877515496670955No Hit
GTAAAA7655530.2261548355488272No Hit
ATAATA7521890.22220692701437625No Hit
AAATTT7414240.2190267986565968No Hit
TAAAAC7014530.20721881810956455No Hit
ATTTAA6904160.2039583372284859No Hit
CAAATA6896740.2037391402715447No Hit
GAAATA6870140.20295333985986858No Hit
AAAACC6843030.20215247335007386No Hit
TCAAAA6670470.19705481473959152No Hit
TATTAA6598240.19492104166683344No Hit
AGAAAA6463160.1909305935612241No Hit
CTAAAA6451350.19058170999498744No Hit
CATAAA6169850.1822658146608963No Hit
TTTAAA6145850.1815568218082562No Hit
TAAACA6144380.18151339599603197No Hit
GATAAA6141240.18142063609781156No Hit
AAGAAA6078440.1795654381334033No Hit
AAAACG6075040.1794649974792793No Hit
AAAGAA6028600.17809309630942072No Hit
AATATT5999900.1772452590231386No Hit
CAATAA5781270.1707866295492759No Hit
TAAAAG5763620.17026522438889682No Hit
AATTTA5727310.16919257728559006No Hit
GAATAA5683180.1678889166777981No Hit
ATAATT5645380.16677225293488995No Hit
TTAAAT5491980.16224060695176531No Hit
AACCAA5458150.16124122244323139No Hit
ACTAAA5379310.15891218092230866No Hit
AATTAT5378880.15889947813369887No Hit
AATCAA5360090.15834439581281937No Hit
GAAATT5316960.1570702765738874No Hit
TACAAA5212490.15398408976908276No Hit
AACTAA5207390.15383342878789674No Hit
TAATTA5163560.15253863059076278No Hit
AAAAGC5147950.1520774898228581No Hit
TATAAT5102980.15074901446522374No Hit
TAACAA5056580.14937829495011953No Hit
TAAATG5055110.14933486913789532No Hit
CAAATT4987290.14733137350180983No Hit
ACAAAT4919570.14533083200261038No Hit
TAATAT4825460.14255069377919538No Hit
CCAAAA4809400.14207625939530372No Hit
TTATAA4797430.14172264921004946No Hit
AAGTAA4771220.14094836993222876No Hit
AAATCA4710670.13915964004775552No Hit
TTAATA4701790.13889731269227867No Hit
TTTTAA4701790.13889731269227867No Hit
AACAAT4679590.13824149430358657No Hit
AATTTT4656100.1375475675490651No Hit
TAATTT4573100.13509563393368473No Hit
AATAAC4559950.1347071649331757No Hit
AATGAA4539720.1341095430411378No Hit
GCAAAA4523330.13362536000552236No Hit
ACCAAA4416540.13047063722496252No Hit
ACAATA4384120.12951290604652116No Hit
AAACTA4354260.12863080077236147No Hit
TAAAGG4305600.12719331776363366No Hit
ACATAA4271260.1261788671569811No Hit
CAAAAC4256390.12573958700203286No Hit
AAGGAA4221340.1247041620234897No Hit
TTAATT4196010.1239558791502658No Hit
TATATA4190960.12380669523752279No Hit
ATTAAT4183980.12360049648287995No Hit
ATCAAA4171140.12322118530671751No Hit
AAATAC4170540.12320346048540151No Hit
TAAAGT4150980.1226256313104998No Hit
AAACAT4114040.12153437314481126No Hit
AAATGG4053320.11974062122763181No Hit
TAAATC4012410.11853208382756905No Hit
TAAACT3973130.1173716988587481No Hit
ATAAAC3942430.11646477883474596No Hit
GAAAAC3861850.11408433533200685No Hit
AGTAAA3860700.11405036275781782No Hit
AATACA3846970.11364475976336998No Hit
TAAAGA3836580.11333782494091452No Hit
TGAAAA3824410.11297830648188828No Hit
AAATGA3785760.11183653257544912No Hit
GTTAAA3749140.11075472764779577No Hit
AAATTG3738510.11044070289681393No Hit
TATATT3693710.10911724957188575No Hit
AAATGT3693290.10910484219696455No Hit
ATATTA3663490.10822450940493641No Hit
ATTATA3607470.10656960192139898No Hit
ATAACA3573040.10555249259154906No Hit
AACATA3549900.10486890531612855No Hit
AATAAG3541670.10462577985041072No Hit
GATTAA3496570.10329346411482453No Hit
GAAAAG3476540.10270175049655866No Hit
CATTAA3450660.10193721987046174No Hit
AAGCAA3444400.10175229090139812No Hit
ATTTTA3418070.10097446665931421No Hit
GGAAAA3415670.10090356737405021No Hit
ATACAA3403800.10055291132568195No Hit
GTAAAT3400580.10045778811795274No Hit
TATTTA3390780.1001682827031247No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)