FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44540055
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1172770.2633068145066278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC296500.035.09049613
ATCGGAA425300.035.0582542
TCGGAAG415400.034.6029933
CGGAAGA407400.034.341964
GATCGGA437550.034.026791
CACGTCT310400.032.82356614
CGTCTGA288950.032.81522816
ACGTCTG296100.032.6604815
GCACACG332700.032.65837511
CACACGT332350.032.46145612
AGAGCAC519350.023.547598
AGCACAC498500.023.17122810
GAACTCC378350.022.89714621
CTGAACT441200.021.17429519
GAAGAGC625650.020.8803626
GAGCACA589100.020.4864279
TCTGAAC501550.019.07308218
TGAACTC488150.018.8858120
AACTCCA492200.017.9559922
GTCTGAA548800.017.80109417
Produced by FastQC (version 0.11.5)