FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36380257
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52650.033.8381243
CGTATGC54900.032.38755444
CTCGTAT51600.032.21883442
ATGCCGT55250.032.05726647
TCTCGTA51400.031.5270841
TATGCCG60150.029.79347246
TGCCGTC65950.026.90665248
ATCTCGT63750.025.63626940
GCCGTCT75900.023.37936449
CCGTCTT79200.022.22857750
CATCTCG78650.020.64601339
CGTCTTC86150.020.5184751
GTATGCC110500.016.66135245
GCTTGAA169650.011.36901259
TGCTTGA200150.09.7417858
GTTCCAT201800.09.48654735
ACAGTTC240600.08.55371532
GTCGTAT14700.07.380915642
CGTCTGA256650.07.36407816
GAACTCC303600.06.674418421
Produced by FastQC (version 0.11.5)