FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32204486
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG42850.030.2221443
CGTATGC43800.029.64654544
CTCGTAT40750.029.37392642
TCTCGTA42350.028.26416841
TATGCCG47450.027.43976246
ATGCCGT47700.027.37085347
AATCTCG47050.024.47135239
TGCCGTC58550.022.41619148
ATCTCGT53900.021.9457140
CCGTCTT60900.021.55136550
GCCGTCT62350.020.99387449
CGTCTTC72900.018.14976351
GTATGCC90850.014.63972645
GCTTGAA144900.09.44566659
GTCAAAT156000.08.68227935
CGTCTGA167150.08.14554716
TGCTTGA173950.07.969035658
CAGTCAA184950.07.6639433
ACGTCTG187800.07.23114515
TCAAATC201450.06.619213636
Produced by FastQC (version 0.11.5)