FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30707779
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG36050.034.370143
CTCGTAT36800.033.19254742
CGTATGC39050.031.72963144
TCTCGTA39250.031.12065941
TATGCCG43000.029.14052646
ATGCCGT45000.027.76923647
ACGGCTA49850.024.99534632
ATCTCGT50500.024.39337240
TGCCGTC52700.024.30751448
CCGTCTT57200.022.089350
CGGCTAC56200.021.85860633
GCCGTCT57650.021.8561349
CATCTCG61850.020.03011339
CGTCTTC67000.018.80789651
TCACGGC74550.016.80739830
GTCACGG77150.016.28602829
GTATGCC85300.014.97706245
AGTCACG84600.014.72774528
CACGGCT85750.014.65296331
GCTTGAA137550.09.74681559
Produced by FastQC (version 0.11.5)