FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36326226
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG65750.042.4269543
CTCGTAT66900.041.64348242
CGTATGC68850.041.3808844
TCTCGTA69050.040.39752241
TATCTCG72300.039.93301439
TATGCCG73350.038.8423946
ATGCCGT73250.038.5158547
TGCCGTC83800.033.9561648
ATCTCGT84450.033.7735140
GCCGTCT89950.031.0897849
CCGTCTT91950.030.71827950
CGTCTTC100550.027.29316151
GTATGCC122350.023.8585345
GCTTATC134400.023.20177736
TATCGCG6250.020.15760639
TAGCTTA177500.018.45541634
ACTAGCT182150.018.02377332
CGCGTAT6750.017.62930742
AGCTTAT184950.017.37122735
GTCACTA193900.017.16556229
Produced by FastQC (version 0.11.5)