FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29136783
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC3875721.3301811665344112TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG498400.060.247443
CGTATGC501400.060.01955444
CTCGTAT483600.059.86710742
TATGCCG501500.059.8261346
TCTCGTA479500.059.7658741
ATGCCGT502550.059.712347
TGCCGTC512500.058.52638248
CGATCAG513250.058.4862933
ACGATCA516800.058.33103632
CCGTCTT513850.058.25723650
ATCTCGT487100.058.25244540
GATCTCG486550.058.2032139
GCCGTCT519500.057.6367149
CACGATC526150.057.2332131
CGTCTTC524100.057.1577451
TCACGAT531500.056.67687230
GTCACGA532900.056.5406229
GTATGCC537150.055.99237445
AGTCACG552200.054.6975628
GCTTGAA588350.051.0425159
Produced by FastQC (version 0.11.5)