FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33490794
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG55750.036.09935443
CTCGTAT56000.034.93697442
CGTATGC60050.034.7383944
ATGCCGT61150.034.0009547
TCTCGTA57550.033.7527541
TATGCCG63300.033.28653346
AATCTCG66450.030.75679839
ATCTCGT72550.027.5437240
TGCCGTC77600.026.92642648
CCGTCTT80650.024.82334550
GCCGTCT87700.022.62817649
CGTCTTC91050.021.22100851
GTATGCC104950.020.47681445
TCGCGTA6900.015.72440141
CGCGTAT7000.015.49976742
CGTCTGA178950.014.76702416
TTGAATC161900.014.526834536
ACGTCTG195250.013.49813815
GCTTGAA157050.013.21709459
GTCACAC214050.012.54112329
Produced by FastQC (version 0.11.5)