FastQCFastQC Report
Thu 29 Aug 2019
H5LJCBGXC_n01_mb237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5LJCBGXC_n01_mb237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26964783
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3042701.1283977327019468TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG360150.064.6470843
CTCGTAT347200.064.4242142
CGTATGC368400.064.2634644
TCTCGTA344100.063.91627541
ATGCCGT369750.063.66259447
TATGCCG373550.063.3025246
TGCCGTC376500.062.3957548
ATCTCGT353450.062.19079640
GCCGTCT374200.061.9561749
CCGTCTT376100.061.8483750
CATCTCG362050.060.7617639
CGTCTTC382550.060.49062351
CGTCTGA412750.059.64582416
ACGTCTG419100.058.80056415
ACACGTC421550.058.67218813
GTATGCC407050.058.35931445
CACGTCT431800.057.11892314
CTTAGGC447550.054.4510133
CACACGT457450.054.12022812
GCACACG466600.053.20855311
Produced by FastQC (version 0.11.5)